Analysis Note

ControlRat brain tissue lysate

Application

Research CategoryNeuroscience

Western Blot: A representative lot detected ASIC1 in WT mice, but demonstrated a loss of signal in ASIC1 knockout mice.

Immunohistochemistry Analysis: A representative lot detected ASIC1 in rat brain tissue (Prof. J. Trimmer, University of California, Davis).

Anti-ASIC1 Antibody, clone N271/44 detects level of ASIC1 & has been published & validated for use in Western Blotting & IHC.

Research Sub CategoryDevelopmental Neuroscience

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Acid-sensing ion channels (ASICs) are broadly distributed in the nervous system where they contribute to certain sensory processes. In particular, they survey moderate decreases in extracellular pH as a means to regulate acid levels and acid-induced pain. ASICs thereby serve as a popular target for the treatment of acidosis. The acid-sensing ion channel 1 consists of a trimeric protein, where each subunit is composed of two transmembrane spanning helices, a highly organized ectodomain, and an intracellular N-terminus and C-terminus.

Immunogen

Epitope: C-terminus

Recombinant protein corresponding to the C-terminus of mouse ASIC1.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blot in rat brain tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected ASIC1 in 10 µg of rat brain tissue lysate.

Specificity

This antibody recognizes the C-terminus of ASIC1.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~60 kDa observed. Two isoforms at ~60 kDa and ~62 kDa are known to exist.